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  • Title: High-performance liquid chromatographic determination of alpha-keto acids in plasma with fluorometric detection.
    Author: Hayashi T, Tsuchiya H, Naruse H.
    Journal: J Chromatogr; 1983 Apr 08; 273(2):245-52. PubMed ID: 6863441.
    Abstract:
    This paper describes a sensitive high-performance liquid chromatographic method for the quantitative determination of alpha-keto acids in plasma using a fluorescence detector. This method is about ten times more sensitive than that reported in a previous paper. Only 50 microliters of plasma are needed for the determination of alpha-keto acids. However, p-hydroxyphenylpyruvic acid could not be analysed because the quinoxalinol derived from it does not exhibit fluorescence.
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