These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Thiol-dependent passive K/Cl transport in sheep red cells: II. Loss of Cl- and N-ethylmaleimide sensitivity in maturing high K+ cells.
    Author: Lauf PK.
    Journal: J Membr Biol; 1983; 73(3):247-56. PubMed ID: 6864777.
    Abstract:
    A fraction of the passive, ouabain-insensitive K+ fluxes in mature low Kr(LK) but not in high K+ (HK) sheep red cells requires the presence of Cl- anions and can be stimulated by volume expansion (Dunham, P.B., Ellory, J.C., J. Physiol (London) 318:511-530, 1981) or treatment with 2 mM N-ethylmaleimide (NEM) (Lauf, P.K., Theg., B.E., Biochem. Biophys. Res. Commun. 92:1422-1428, 1980). In the present study it is shown that reticulocytes of both anemic LK and HK sheep possess the Cl- -dependent K+ transport system which subsequently remains functional in mature LK but not in HK red cells. Kinetically, Cl- -mediated K+ fluxes of reticulocytes of LK sheep are different from mature red cells only in their Vmax values as measured in Na+ or choline+ media, while there is an apparently much lower affinity for external K+ ions in reticulocytes of HK sheep. N-ethylmaleimide stimulated K+ transport in the reticulocytes of both sheep genotypes suspended in Cl- but failed to do so in NO3- media. The data are interpreted in terms of their biochemical, physiologic, and genetic implications for the HK/LK transition in sheep red cells.
    [Abstract] [Full Text] [Related] [New Search]