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  • Title: 3-O-methyl-D-glucose uptake in isolated rat hepatocytes. Effects of dexamethasone.
    Author: Madar Z, Felig P.
    Journal: Mol Pharmacol; 1983 Jan; 23(1):141-5. PubMed ID: 6865898.
    Abstract:
    We examined the uptake of 3-O-methyl-D-glucose, a nonmetabolizable hexose, by isolated rat hepatocytes. The uptake of 3-O-methyl-D-glucose was linear for 1 min at 22 degrees, and Lineweaver-Burk analysis demonstrated an apparent Km of approximately 6 mM. Cytochalasin B (40 microM) and phloridzin (2 mM) inhibited 3-O-methyl-D-glucose uptake by 88% and 63%, respectively. D-Glucose (20 mM) inhibited the initial rate of 3-O-methyl-D-glucose uptake by 55% (p less than 0.001), whereas L-glucose was without any significant effect. The uptake of 3-O-methyl-D-glucose remained unchanged in the presence of Na+ (0-150 mM) in the incubation medium. After 30 min dexamethasone inhibited glucose uptake (the maximal effect being achieved in a time- and concentration-dependent manner) at 2 microM and 0.5 microM concentrations by 50% and 25%, respectively. Dexamethasone produced a decrease in the Vmax but did not change the Km. Insulin, glucagon, gastric inhibitory polypeptides, and pancreozymin had no effect on 3-O-methyl-D-glucose uptake in isolated hepatocytes. These findings are consistent with the conclusion that 3-O-methyl-D-glucose uptake in isolated rat hepatocytes occurs via a stereospecific, carrier-mediated, facilitated diffusion process. Dexamethasone decreases this process of facilitated diffusion in the isolated hepatocyte.
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