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  • Title: Enzyme-linked immunosorbent assay for leucine and methionine enkephalins.
    Author: Zamboni G, Jones CA, Hughes J.
    Journal: Anal Biochem; 1983 Apr 01; 130(1):83-7. PubMed ID: 6869812.
    Abstract:
    An enzyme-linked immunosorbent assay for enkephalins was developed by coupling the peptides to a carrier molecule (bovine serum albumin) in order to allow the antibody-antigen reaction to take place in the solid phase. The assay was shown to be highly reproducible. Its sensitivity was 14 nmol/liter for leucine enkephalin and 27 nmol/liter for methionine enkephalin, which is similar to that obtained when the same antibodies were used in radioimmunoassay.
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