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  • Title: Effects of methylcholanthrene and benzanthracene on blastogenesis and aryl hydrocarbon hydroxylase induction in splenic lymphocytes from three inbred strains of mice.
    Author: Alfred LJ, Wojdani A.
    Journal: Int J Immunopharmacol; 1983; 5(2):123-9. PubMed ID: 6874165.
    Abstract:
    The effects of polycyclic aromatic hydrocarbons (PAH) such as benzanthracene (BA) and methylcholanthrene (MCA) on 3H-thymidine incorporation and aryl hydrocarbon hydroxylase (AHH) induction were assayed in mitogen-activated and non-activated splenic lymphocyte cultures derived from three strains of mice (C57BL, C3H and DBA/2). Results of three separate experiments on blastogenesis and the substrate induction of AHH were statistically significant. In mitogen-activated and non-activated lymphocytes from C57BL and C3H mice (AHH responsive strains) the percentage of blastogenesis induced by BA or MCA was higher than in lymphocytes from the non-responsive strain (DBA/2). On the basis of the PAH concentrations used MCA and BA were similar as inducers of blastogenesis and of AHH activity. AHH induction was measurable only in mitogen-activated lymphocytes and showed a non-linear relation to blastogenesis. In responsive strains, 10 microM oF BA or 1.5 microM of MCA induced AHH 2-5 fold, while in non-responsive mice induced AHH was very close to the basal level. This difference between the level of induction of lymphoblast formation and AHH in responsive and non-responsive strains of mice may be related to different subpopulations of lymphocytes in spleen or other lymphatic organs.
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