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  • Title: [Commitment to erythroid differentiation and globin gene expression in the absence of heme accumulation in Friend leukemia cells].
    Author: Gambari R.
    Journal: Boll Soc Ital Biol Sper; 1983 May 30; 59(5):631-7. PubMed ID: 6882562.
    Abstract:
    Friend leukemia cells (FLC) induced to erythroid differentiation by 5 mM hexamethylenebisacetamide (HMBA) are suitable for studies focused on the expression of different genes involved in the same differentiative program. In our studies FL cells have been cultured in alpha-medium (Gibco), 10% fetal calf serum (FCS, Flow Laboratories). Erythroid differentiation has been analysed by benzidine staining of the cells and globin synthesis by pulse-labelling the cells with 3H-leucine (New England Nuclear) and electrophoresis of newly-synthesized proteins on polyacrylamide gels. Hemoglobin production was determined by cellulose acetate gel electrophoresis. Our results demonstrate that heme synthesis is suppressed by the iron-chelator desferrioxamine. In addition "commitment" to erythroid differentiation and transcription and translation of globin mRNAs occur in HMBA-treated FLC in the absence of heme accumulation.
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