These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: R-banding of human chromosomes by heat denaturation and Giemsa staining after amethopterin-synchronization.
    Author: Richer CL, Murer-Orlando M, Drouin R.
    Journal: Can J Genet Cytol; 1983 Jun; 25(3):261-9. PubMed ID: 6883179.
    Abstract:
    Human late prophase to late metaphase chromosomes were prepared after amethopterin cell synchronization. R-banding was produced by heat denaturation followed by Giemsa staining (RHG). Haploid sets of prophase chromosomes contain approximately 850 bands. Sequences of chromosomes of different degrees of condensation are presented; their analysis provides helpful information to identify the elongated chromosomes and to follow band subdivision. The heat denaturation technique is free from most of the disadvantages encountered with R-banding by 5-bromodeoxyuridine incorporation. Giemsa stained R-bands produced by heat denaturation on prophase and prometaphase chromosomes are useful to analyse the numerous chromosome anomalies involving R-bands. In conjunction with G-banding, it is also important to compare adequately the positive and negative regions of each chromosome to define the anomalies with precision.
    [Abstract] [Full Text] [Related] [New Search]