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  • Title: Cloning of genomic sequences from the human Y chromosome after purification by dual beam flow sorting.
    Author: Müller CR, Davies KE, Cremer C, Rappold G, Gray JW, Ropers HH.
    Journal: Hum Genet; 1983; 64(2):110-5. PubMed ID: 6885043.
    Abstract:
    Human Y chromosomes were purified by dual beam flow sorting from a human X Chinese hamster cell line retaining the Y as the only free human chromosome. DNA was extracted from the Y fraction and cloned into lambda gtWES . lambda B vector arms. More than 100 recombinant clones carrying human inserts have been characterised by Benton-Davis plaque screening and Southern blotting or in situ hybridisation. Several repetitive sequences were found to be predominantly located on the Y, whereas the majority also cross-hybridised with autosomal DNA. One repetitive clone gave a specific hybridisation signal with the X and the Y chromosome but not with autosomes. Preliminary evidence indicates that many clones contain single copy as well as repetitive sequences. However, no Y-specific single copy sequence has yet been identified.
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