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  • Title: Direct solid-phase 125I radioimmunoassay of serum cortisol.
    Author: Brock P, Eldred EW, Woiszwillo JE, Doran M, Schoemaker HJ.
    Journal: Clin Chem; 1978 Sep; 24(9):1595-8. PubMed ID: 688623.
    Abstract:
    We report a solid-phase iodine- 125 radioimmunoassay for serum cortisol in which the interfering binding proteins are inactivated by a combination of reaction pH and 8-anilino-1-naphthalenesulfonic acid. The procedure is easy to perform and gives accurate and reliable results. The shift in pH for optimum binding of an antibody resulting from immobilization on a solid support, used to decrease cortisol binding competition in the present test, is potentially exploitable in other antibody and enzyme systems.
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