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  • Title: Emergency gas-chromatographic assay of phenobarbital and phenytoin and liquid-chromatographic assay of theophylline.
    Author: Shihabi ZK.
    Journal: Clin Chem; 1978 Sep; 24(9):1630-3. PubMed ID: 688630.
    Abstract:
    Phenobarbital and phenytoin are extracted and concentrated in a single step, without solvent evaporation, followed by on-column methylation and gas-chromatographic quanitation. A similar extraction step for the assay of theophylline by high-pressure liquid chromatography is described. The extraction step can be completed in less than 2 min, the chromatographic step in less than 8 min. This extraction method yields clean chromatograms, avoids evaporating health-hazardous solvents, and is applicable to other drugs.
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