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  • Title: Isolation and characterization of a Chlamydomonas L-asparaginase.
    Author: Paul JH.
    Journal: Biochem J; 1982 Apr 01; 203(1):109-15. PubMed ID: 6896642.
    Abstract:
    An L-asparaginase (EC 3.5.1.1) specific for L-asparagine has been purified from a marine Chlamydomonas species, the first such enzyme to be purified from a microalga. The purified enzyme (mol.wt. 275 000) possessed a Km for asparagine of 1.34 x 10(-4) M and showed limited antitumour activity in an antilymphoma assay in vivo. Properties of the enzyme are contrasted with those of asparaginases from prokaryotic and eukaryotic sources.
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