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  • Title: The expression of HLA on two cultured choriocarcinoma cell lines.
    Author: Tanimoto M, Takahashi T, Nishizuka Y.
    Journal: Gan; 1982 Feb; 73(1):27-34. PubMed ID: 6956527.
    Abstract:
    Since gestational choriocarcinoma is believed to be derived from trophoblast, paternal antigens are expected to be present on carcinoma cells. Two choriocarcinoma lines (GCC-SV and Lu-75) transplantable in nude mice were obtained and cultures were started in order to study the expression of HLA on these tumor cells. To date, two human chorionic gonadotropin-producing cell lines, designated GCC-SV(c) and Lu-75(c), have been maintained for over two years with more than 15 passages and have been employed for serological studies. First, GCC-SV(c) (patient A2, AW30, B8, B10; husband A3, AW26, BW16, BW35) was tested by protein A assay with HLA-A typing sera and anti-beta 2 microglobulin; the phenotype was A2+, A3-, AW26-, beta 2+, which corresponded to HLA-A type of the patient. In contrast, HLA-A was not demonstrated on Lu-75(c) cells (patient A2, BW35, BW39; husband A9, AW26, BW22) by the direct test, but anti-beta 2 microglobulin serum gave a positive reaction. A mouse monoclonal antibody against HLA was also employed, but it did not react against Lu-75(c) cells. Further serological analysis was carried out by means of absorption tests, and it was confirmed that GCC-SV(c) was A2+, A3-, AW26- and Lu-75(c) was A2-, A9-, AW26-. The results so far obtained do not demonstrate the expression of paternal HLA-A antigens on choriocarcinoma cells.
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