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  • Title: Ribosomal protein S20 purified under mild conditions almost completely inhibits its own translation.
    Author: Wirth R, Littlechild J, Böck A.
    Journal: Mol Gen Genet; 1982; 188(1):164-6. PubMed ID: 6960227.
    Abstract:
    The efficiency of ribosomal protein S20 to act as repressor of its own synthesis in an in vitro system was found to depend greatly on the procedures employed to purify this protein. Whilst conventionally purified r-protein S20 inhibited its own synthesis by some 30%, up to 90% inhibition was observed if "milder" purification conditions were used. Evidence is presented that the latter preparation shows also a higher binding affinity to 16S rRNA.
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