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  • Title: The stereospecificity of 3 alpha- and 12 alpha-bile salt hydroxysteroid dehydrogenase systems from four microbial sources.
    Author: Macdonald IA, Chang FC.
    Journal: Enzyme; 1982; 28(4):392-5. PubMed ID: 6961034.
    Abstract:
    5 beta-Cholanoates, having a hydroxyl group in the 3 alpha and 3 beta and/or 12 alpha and 12 beta configurations, were tested as substrates for two preparation of 3 alpha-hydroxysteroid dehydrogenase (HSDH) and two preparations of 12 alpha-HSDH. When the 3-OH group was in the alpha configuration, both 3 alpha-HSDH preparations reacted, but when it was in the beta configuration, neither 3 alpha-HSDH preparations reacted. This also held true for the 12 alpha-HSDH preparations.
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