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  • Title: The preparation and analysis of myelin from small quantities of central nervous tissue: regional studies of the quaking mouse.
    Author: Fagg GE, Waehneldt TV, Neuhoff V.
    Journal: Adv Exp Med Biol; 1978; 100():135-45. PubMed ID: 696471.
    Abstract:
    Myelin of considerable purity may be isolated from small (minimum 1 mg wet weight) samples of central nervous tissue, using a 4-step centrifugation procedure. The separation of myelin proteins by micro-linear gradient polyacrylamide gel electrophoresis yields similar results to those obtained by macro-scale (homogeneous) gel systems. These techniques have been employed for a preliminary study of the regional composition of myelin fractions from the Quaking mouse.
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