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Title: T lymphocytes in colostrum and peripheral blood differ in their capacity to form thermostable E-rosettes. Author: Richie ER, Steinmetz KD, Meistrich ML, Ramirez I, Hilliard JK. Journal: J Immunol; 1980 Nov; 125(5):2344-6. PubMed ID: 6968780. Abstract: The cellular population of human breast milk collected within 5 days postpartum consists primarily of lipid laden polymorphonuclear leukocytes and macrophages, whereas lymphocytes comprise < 10% of the total cells. A discontinuous density gradient technique was developed to obtain highly enriched (75 +/- 6%) fractions of milk lymphocytes. The majority of milk lymphocytes are T cells as determined by their capacity to form rosettes at 4 degrees C with sheep erythrocytes (E). The lymphocytic origin of the rosetted cells was confirmed by morphologic evaluation of Wright-Giemsa-stained cytocentrifuge slides. A novel finding is that a large proportion of milk T lymphocytes form E-rosettes at 37 degrees C as well as at 4 degrees C. The capacity to form thermostable E-rosettes is not a general property of T lymphocytes in the postpartum period, since peripheral blood T lymphocytes from milk donors form few thermostable E-rosettes. In addition, peripheral blood lymphocytes did not acquire the capacity to form thermostable E-rosettes after density gradient centrifugation nor after incubation with autologous milk. The finding that T lymphocytes in milk form thermostable E-rosettes suggests that milk T lymphocytes may be activated or immature. In either case, these data support the concept that lymphocytes that home to the mammary gland are a select subset of the total lymphocyte population.[Abstract] [Full Text] [Related] [New Search]