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  • Title: Reliability of alpha-naphthyl-acetate esterase staining of blood smears for the enumeration of circulating human T lymphocytes.
    Author: Ferrari FA, Maccario R, Marconi M, Vitiello MA, Ugazio AG, Burgio V, Siccardi AG.
    Journal: Clin Exp Immunol; 1980 Aug; 41(2):358-62. PubMed ID: 6969154.
    Abstract:
    Cytocentrifuged preparations of glutaraldehyde-fixed E rosettes were stained for acid alpha-naphthyl-acetate esterase activity (ANAE). On average, 84% of the rosette-forming cells (E+) also stained positively for the enzyme activity (A+). ANAE staining of blood smears of thirty-three healthy controls was compared to E rosetting by a conventional method. A+ and E+ fractional values are highly significantly correlated; the regression of E+ on A+ is E+ = 0.805.A+ + 16.8 (P < 10(-6)). Since the correlation between E+ and A+ cells is both statistically and cytologically significant, we propose blood smear ANAE staining as a simple and convenient method to enumerate circulating T lymphocytes in clinical practice.
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