These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Isolation and partial characterization of a 72,000-dalton glycoprotein (Tgp72) on human thymus and T cells: possible relationship to mouse Ly-1 antigens.
    Author: Ishii Y, Fujimoto J, Koshiba H, Kikuchi K.
    Journal: J Immunol; 1981 Jun; 126(6):2171-6. PubMed ID: 6971888.
    Abstract:
    A xenoantiserum was raised in guinea pigs to a human T cell antigen (Tgp72) partially purified from cell membrane of T cell leukemia cells from a patient with chronic lymphosarcoma cell leukemia of T cell-type by using sequential chromatographic procedures. The antiserum reacted with all of human thymus and peripheral T cells as well as with a variety of cultured leukemic T cell lines, but not with B cells, granulocytes, and other cultured lymphoid cell lines, including normal B cell, Burkitt, myeloid, and non-T non-B cell lines. Immunoprecipitated material from 125I-labeled thymocyte, T cell, and T cell leukemia cell-surface glycoproteins by this antiserum was analyzed by SDS-polyacrylamide gel electrophoresis, which disclosed a single component with a m.w. of 72K (Tgp72). Further immunochemical data suggest that Tgp72 antigen on human thymus and T cells is an acidic glycoprotein composed of a single polypeptide rather than sulfhydryl-linked subunits, showing a marked electric charge heterogeneity in DEAE anion-exchange resin chromatography. This is further confirmed by the data obtained from 2-dimensional polyacrylamide gel electrophoresis, indicating that Tgp72 is a 72K m.w. glycoprotein with an isoelectric point ranging from 4 to 7. Because of its similarity in tissue distribution and immunochemical characteristics, Tgp72 on human thymus and T cells was assumed to be a human homologue of Ly-1 antigens in murine system.
    [Abstract] [Full Text] [Related] [New Search]