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  • Title: [Assay of serum 24,25-dihydroxycholecalciferol by a competitive protein binding method].
    Author: Coen G, Bianchi AR, Ballanti P, Bianchini G, Bravo E, Mazzaferro S, Cinotti GA.
    Journal: Boll Soc Ital Biol Sper; 1981 Sep 30; 57(18):1880-6. PubMed ID: 6976177.
    Abstract:
    24-25(OH)2 D3 is a vitamin D metabolite due to renal 24-hydroxylation of 25OHD3. The metabolite has some important biological functions on the bone and intestine. Its affinity to the D serum binding protein allows a competitive binding protein assay to be carried out as for 25OHD3, following a chromatographic purification step on Sephadex LH 20. Serum levels of the metabolite in 18 control subjects were found to be 2,69 +/- 1,34 ng/ml while 25OHD3 was 17,41 +/- 7,39 ng/ml and the ratio 24-25(OH)2D3 /25OHD3 in the serum was 0,15. The assay of 24-25(OH)2D3 is useful for physiological studies and for identification of vitamin D depletion states.
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