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  • Title: Circulating immune complexes in Behçet's syndrome: purification, characterization and cross-reactivity studies.
    Author: Valesini G, Picardo M, Pastore R, Pivetti P, Serafini U.
    Journal: Clin Exp Immunol; 1981 Jun; 44(3):522-7. PubMed ID: 6976862.
    Abstract:
    The C1q-binding assay was performed on 30 sera from patients with Behçet's syndrome and circulating immune complexes were found in 46%. Circulating immune complexes were isolated and purified from the sera of two patients by immunoadsorption on a column of polymethylmetacrylate beads coated with C1q and then labelled with 125I. In double immunodiffusion these purified immune complexes were found to contain IgG, C1q, C1s and C3. Anti-IgG activity was not detectable in the purified immune complexes while specific cross-reactivity was found in a solid-phase radioimmunoassay with the majority of the sera of behçet's syndrome.
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