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  • Title: Isoelectric focusing studies on Mycobacterium chelonei.
    Author: Sparks J, Ross GW.
    Journal: Tubercle; 1981 Dec; 62(4):289-93. PubMed ID: 6977905.
    Abstract:
    Analytical IEF has been used to give a direct visual comparison of beta-lactamases, down to extremely low levels, from very crude intracellular preparations. Identity and non-identity of strains could be proved by the pattern of bands produced by their beta-lactamases. Mycobacterial species gave a variety of beta-lactamase patterns. Identity was established between some strains of M. chelonei. IEF distinguished between enzymes within both the chelonei and abscessus sub-species that could not be differentiated by other methods. This technique could provide a means of identifying the source of a M. chelonei infection.
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