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  • Title: A new method of preparation and some properties of 3-hydroxyproline.
    Author: Szymanovicz G, Mercier O, Randoux A.
    Journal: Biochimie; 1978 Sep 04; 60(5):499-503. PubMed ID: 698288.
    Abstract:
    The hydrolyzate of Delonix Regia seed extract is fractionated sequentially on Dowex 50 X 8 resin and on QAE Sephadex A 25. Purification is completed by recrystallisation from ethyl alcohol. 3-hydroxyproline is destroyed by NO2H and by chloramine T, which prevents from using most of the colorimetric reactions in use for 4-hydroxyproline. For its characterization, 3-hydroxyproline may be clearly separated from 4-hydroxyproline by TLC chromatography and by high voltage paper electrophoresis.
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