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  • Title: Induction of mutations in specific genes of bacteriophage T4 using cloned restriction fragments and marker rescue.
    Author: Völker TA, Showe MK.
    Journal: Mol Gen Genet; 1980 Feb; 177(3):447-52. PubMed ID: 6990202.
    Abstract:
    Saturation of a specific region of a chromosome with conditional lethal mutations becomes increasingly difficult as the genome size of the organism increases. We show that mutagenesis of cloned genes followed by their re-integration into a non-mutagenized organism is a practical way to circumvent this difficulty.
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