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  • Title: Immunochemical assay of rat postheparin plasma triacylglycerol lipases.
    Author: Kuusi T, Ehnholm C, Nikkilä EA.
    Journal: Atherosclerosis; 1980 Apr; 35(4):363-74. PubMed ID: 6990938.
    Abstract:
    Immunochemical methods for selective measurement of lipoprotein lipase and hepatic lipase activities in rat postheparin plasma are described and validated. Lipoprotein lipase was measured using a substrate containing 10% serum and 0.1 M NaCl after inactivation of hepatic lipase with a specific antiserum. Hepatic lipase was measured at 1.0 M NaCl with a serum-free substrate. The heparin dose-response curve indicated maximum relase of both activities at a heparin dose of 500 IU/kg. The lipase activities in rat postheparin plasma were 3 to 4-fold higher than those in human postheparin plasma. The LPL activity in female rats was significantly higher than in males whereas there was no sex difference for hapatic lipase.
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