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  • Title: [Applied gene technology using biological N2-fixation for an example (author's transl)].
    Author: Pühler A.
    Journal: Arzneimittelforschung; 1980; 30(3a):570-5. PubMed ID: 6992791.
    Abstract:
    About biological nitrogen fixation most information on the genetic and enzymatic level is known from investigations with Klebsiella pneumoniae. The gene region for N2-fixation (nif) was transferred from K.pneumoniae to the enterobacterium Escherichia coli. After acquisition of nif E.coli is able to fix nitrogen. Plasmid pRD1 was constructed which carries the nif-region. Using genetic engineering techniques the exact location of nif on pRD1 was determined. Subsequently the nif-region comprising 7 operons with 14 known genes was cloned on a multicopy plasmid vector. The use of the Klebsiella-nif-region in biotechnology is discussed. Difficulties arising in experiments designed to construct N2-fixing plants are mentioned.
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