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  • Title: Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.
    Author: Gallwitz D, Sures I.
    Journal: Proc Natl Acad Sci U S A; 1980 May; 77(5):2546-50. PubMed ID: 6994099.
    Abstract:
    The complete nucleotide sequence of the actin gene from Saccharomyces cerevisiae has been determined. The coding region is interrupted by a 304-base-pair intervening sequence that is located within the triplet coding for amino acid 4. DNA sequences of the intron-exon junctions are similar to those found in higher eukaryotes and can be aligned such that the intron starts with the dinucleotide 5'-G-T-3' and ends with 5'-A-G-3'. Regions fo homology within the sequences upstream from the initiation codon and those following the termination codon have been detected between the yeast iso-1-cytochrome c gene and the actin gene. As deduced from the nucleotide sequence, yeast actin has 374 amino acid residues. Its primary structure, especially the NH2-terminal third of the protein, is highly conserved during evolution.
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