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  • Title: (A14-phenylalanine)insulin: a new synthetic analogue.
    Author: Danho W, Sasaki A, Büllesbach E, Gattner HG, Wollmer A.
    Journal: Hoppe Seylers Z Physiol Chem; 1980 May; 361(5):747-54. PubMed ID: 7000657.
    Abstract:
    An analogue of porcine insulin which differs from the native molecule in that the amino acid residue A14-tyrosine is replaced by phenylalanine has been synthesized. The [PheA14]A chain was synthesized by the fragment condensation method and purified as tetra-S-sulfonate by ion-exchange chromatography on DEAE-cellulose at pH 5.6. Conversion of the tetra-S-sulfonate A chain to the sulfhydryl form and combination with native porcine sulfhydryl B chain gave the [PheA14]insulin, which was purified by gel filtration and ion-exchange chromatography on DEAE-cellulose. The biological activity of this analogue was 96 +/- 6% as measured by the rat epididymal adipocytes. This shows that A14-tyrosine is not essential for the biological activity of the hormone.
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