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  • Title: Construction and characterization of a cDNA clone containing a portion of the bovine prolactin sequence.
    Author: Nilson JH, Thomason AR, Horowitz S, Sasavage NL, Blenis J, Albers R, Salser W, Rottman FM.
    Journal: Nucleic Acids Res; 1980 Apr 11; 8(7):1561-73. PubMed ID: 7001369.
    Abstract:
    Poly(A)-containing RNA from the bovine anterior pituitary has been used as a template for the enzymatic synthesis of double-stranded cDNA. The resulting double-stranded cDNA was inserted into the Pst I site of pBR322 with the oligo(dG)-oligo(dC) tailing technique and subsequently cloned in E. coli chi 1776. Clones containing sequences complementary to prolactin mRNA were identified by colony hybridization with partially purified prolactin cDNA. A 250 base pair sequence from one prolactin positive clone was extensively characterized and shown to contain the coding information for amino acids 119-192 of authentic bovine prolactin. The recombinant DNA from this clone was covalently attached to diazotized aminocellulose and used to purify prolactin mRNA from a mixture of mRNAs.
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