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  • Title: Triazine dyes, a new class of affinity labels for nucleotide-dependent enzymes.
    Author: Clonis YD, Lowe CR.
    Journal: Biochem J; 1980 Oct 01; 191(1):247-51. PubMed ID: 7008786.
    Abstract:
    A number of reactive dichlorotriazine dyes specifically and irreversibly inactivate pig heart lactate dehydrogenase, yeast glucose 6-phosphate dehydrogenase and yeast hexokinase at sites competitive with NAD+, NADP+, and ATP respectively. Monochlorotriazine dyes, including Cibacron Blue F3G-A, do not inactivate lactate dehydrogenase but display high affinity and thus inhibit the inactivation by dichlorotriazine dyes. These data are interpreted in terms of the ability of nucleotide-binding enzymes to bind polysulphonated aromatic chromophores.
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