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Pubmed for Handhelds
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Title: Description of a coverslip holder for, and observations on, the critical point drying of cell cultures. Author: Evers P, Robinson K, Maistry L, Higginson E. Journal: J Microsc; 1980 Nov; 120(Pt 2):225-8. PubMed ID: 7012363. Abstract: The elimination of artefact during the preparation of cell cultures for scanning electron microscopy is difficult. Collapse of cellular projections, cytoplasmic cracks, perforations and fracturing of cell-cell processes and cell-substrate attachments occur during fixation, dehydration and critical point drying. Coating and storage may cause further artefact. A specimen holder which serves to minimize turbulence in the critical point dryer and which allows for the simultaneous processing of up to five coverslips, as well as a reproducible technique for the preparation of cell cultures are described.[Abstract] [Full Text] [Related] [New Search]