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  • Title: Hormonal requirements for growth of arterial smooth muscle cells in vitro: and endocrine approach to atherosclerosis.
    Author: Weinstein R, Stemerman MB, Maciag T.
    Journal: Science; 1981 May 15; 212(4496):818-20. PubMed ID: 7013068.
    Abstract:
    In this study the hormonal requirements for the growth of arterial smooth muscle cells in vitro were determined. A serum-free, biochemically defined medium, supplemented with the relevant hormones, permitted proliferation and propagation of normal diploid mammalian arterial smooth muscle cells. Serum-free, hormone-supplemented cultures spontaneously formed atherosclerotic plaque-like nodules. Thus atherosclerosis may be mediated by a complex endocrine system.
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