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  • Title: Mechanistic deductions from isotope effects in multireactant enzyme mechanisms.
    Author: Cook PF, Cleland WW.
    Journal: Biochemistry; 1981 Mar 31; 20(7):1790-6. PubMed ID: 7013799.
    Abstract:
    In the enzymatic mechanism with two or more substrates, comparison of the isotope effects on the maximum velocity and on the apparent V/K values when each substrate concentration is varied allows one to deduce the kinetic mechanism and obtain quantitative information on the relative rates at which substrates dissociate from the enzyme, as opposed to undergoing reaction to give products. Theory is also presented for using the effects of other reactants on the apparent isotope effects determined by the equilibrium perturbation method to determine the same information. With liver alochol dehydrogenase, DPN is not released at an appreciable rate from the E-DPN-cyclohexanol complex, while cyclohexanol is released much more rapidly than it reacts to give products, so that the mechanism appears ordered. With DPNH and cyclohexanone, however, the reaction is random since DPNH can be released from the ternary complex at a finite rate. With yeast alcohol dehydrogenase, acetone, when present, prevents DPNH release from the enzyme so that the mechanism at equal rates from E-DPN-2-propanol so tht the reaction is random in this direction.
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