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  • Title: Isolation of an azide mutagenic metabolite in Salmonella typhimurium.
    Author: Owais WM, Kleinhofs A, Ronald RC, Nilan RA.
    Journal: Mutat Res; 1981 May; 91(3):155-61. PubMed ID: 7017392.
    Abstract:
    A scheme that employs a cation-exchange column and high-pressure liquid chromatography (HPLC) is devised to isolate and process large quantities of azide metabolite produced by S. typhimurium TA1530 strain. The mutagenic metabolite adheres strongly to the cation-exchange column, thus providing a convenient way to separate the metabolite from unreacted azide (N3-). The metabolite is very polar and only sparingly soluble in most organic solvents. Recrystallization in a methanol-carbon tetrachloride solvent system gave rise to microcrystalline material that decomposes with charring and gas evolution at 173-176 degrees C. The infrared spectrum indicates the presence of a covalently bound azide moiety.
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