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  • Title: Total synthesis of tRNAfMet.
    Author: Ohtsuka E, Markham AF, Tanaka S, Tanaka T, Miyake T, Nakagawa E, Wakabayashi T, Taniyama Y, Fujiyama K, Nishikawa S, Fukumoto R, Uemura H, Doi T, Tokunaga T, Ikehara M.
    Journal: Nucleic Acids Symp Ser; 1980; (7):335-43. PubMed ID: 7019860.
    Abstract:
    Oligonucleotides corresponding to the E. coli tRNAfMet fragments and their analogs have been synthesized by the phosphodiester or phosphotriester method. The nascent fragments were joined by using RNA ligase to yield a total tRNA molecule. The synthetic molecule was tested its ability to accept methionine in the presence of purified methionyl-tRNA synthetase from E. coli. The charged tRNA and the hydrolyzed methionine were isolated by gel filtration. The discharged tRNA was characterized by nearest neighbor analysis and 5'-end analysis.
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