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  • Title: A method for rapid determination of IgG containing circulating immune complexes using polyethyleneglycol and radioactively labeled protein A.
    Author: Stevens WJ, Bridts C.
    Journal: Immunol Lett; 1981 Apr; 3(1):1-4. PubMed ID: 7024111.
    Abstract:
    A simple method for rapid determination of IgG containing circulating immune complexes by commercially available reagents was developed. In this method, serum is incubated with 2.5% polyethyleneglycol. After washing, the precipitate is incubated with radioactively labeled protein A, which binds to IgG in the immune complex. After a further washing, the radioactivity bound is measured. Artificially formed complexes of heat-aggregated human IgG are diluted and a reference curve is constructed. This method is compared to the solid phase C1q-binding method. Our method is slightly more sensitive than the C1q-binding method.
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