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  • Title: Preparation of small temporal bones for high-resolution light microscopy.
    Author: Chole RA, Charpied GL.
    Journal: Arch Otolaryngol; 1981 Oct; 107(10):610-2. PubMed ID: 7025814.
    Abstract:
    A technique was used for rapid processing of small temporal bones for high-resolution light microscopy, Temporal bones were decalcified in edetic acid and embedded in glycol methacrylate. Sections (thickness, 1 micrometer) were stained with a variety of stains that demonstrated cellular details not usually seen with conventional methods. This technique is suitable for routine histologic, histochemical, and radioautographic studies of small temporal bones.
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