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  • Title: [Identification and the physicochemical characteristics of the extrachromosomal DNA from a Streptomyces antibioticus strain].
    Author: Orlova VA, Bogush VG, Sharygin AA, Danilenko VI.
    Journal: Antibiotiki; 1981 Sep; 26(9):655-9. PubMed ID: 7027917.
    Abstract:
    The data on centrifugation of DNA in the density gradient of caesium chloride-ethidium bromide, electron microscopy and electrophoresis of the DNA restricts from the satellite gradient band indicate that Str. antibioticus, the oleandomycin-producing organism possessed extrachromosomal cyclic DNA with the molecular weight of 21.3. 10(6) +/- +/- 0.3 10(6) daltons. The extrachromosomal cyclic DNA had 7 identification sites for endonuclease BamHI, 5 sites for PstI, 12 sites for PvuII, more than 14 sites for SmaI and non for endonucleases Eco RI and HindIII.
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