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  • Title: [Immunochemical isolation and characteristics of polyribosomes synthesizing rat serum albumin].
    Author: Borovkov AIu, Krylova IN, Sviridov SM.
    Journal: Mol Biol (Mosk); 1981; 15(5):1040-50. PubMed ID: 7029242.
    Abstract:
    A novel one-step procedure is described for purification of a specific class of polysomes. The analysis of optimal conditions for immunoadsorption of albumin-synthesizing polysomes was carried out. The native albumin-synthesizing polysomes were isolated. This preparation has the sedimentation coefficient similar to that of the class revealed amongst polysomes from rat liver with 125I- and peroxidase-labeled antibodies. We translated albumin-synthesizing polysomes in oocytes. The analysis of the product of translation of the preparation of albumin-synthesizing polysomes shows that the albumin makes up 90% of the exogenous synthesis.
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