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  • Title: An enzyme immunoassay for serum tobramycin.
    Author: Kominami G, Nakamura M, Mori S, Kono M.
    Journal: Clin Chim Acta; 1981 Dec 09; 117(2):189-97. PubMed ID: 7030524.
    Abstract:
    A heterogeneous enzyme immunoassay for serum tobramycin was developed using tobramycin-beta-D-galactosidase conjugate as the labelled antigen and 4-methylumbelliferyl-beta-D-galactoside as the fluorescence substrate. B/F separation was carried out using an immobilized second antibody, Immunobead. The minimal detectable level was 10 pg/tube or 0.2 microgram/ml of serum. Assay recovery was excellent and the intra-assay and inter-assay variances were 4.41% and 12.6%, respectively. The values from the enzyme immunoassay agreed well with those from radioimmunoassay and bioassay. This assay system is highly sensitive and can be easily applied to other drugs and hormones.
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