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  • Title: [Establishment of the endocrine function of cultured beta cells of the islets of Langerhans].
    Author: Komissarenko VP, Turchin IS, Lazareva NP.
    Journal: Biull Eksp Biol Med; 1981 Oct; 91(10):489-91. PubMed ID: 7032623.
    Abstract:
    Monolayer cultures of pancreatic islets obtained from the pancreas of guinea-pigs aged 10 days and 3-3.5-months pig embryos were studied at the 2d-3d, 5th-6th and 15th-16th days. These times corresponded with the stages of culture intense growth, stabilization and physiological dying off. The rate of insulin biosynthesis and secretory activity of beta-cells were evaluated from the intensity of 3H-uridine and 3H-leucine incorporation and from the magnitude of immunoreactive insulin secretion into the cultural medium. The proliferating and insulin-synthetizing beta-cells were detected by selective 3H-thymidine or 3H-leucine incorporation. In the stages of culture intense growth and dying off the viable beta-cells were distributed into insulin-synthesizing and proliferating ones. During the transition to the stabilization stage the proliferative level of beta-cells drastically decreased, while the biosynthesis and insulin secretion rates increased. This manifested in the increased number of beta-cells which intensely incorporated 3H-thymidine into the nucleus, and 3H-leucin into the cytoplasm, as well as in the rise of immunoreactive insulin concentration in the culture.
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