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  • Title: [Protein yield in the rehydration of dehydrated Saccharomyces cerevisiae yeast cells].
    Author: Popova MV, Laĭvenieks MG, Damberg BE, Kulaev IS, Beker ME.
    Journal: Mikrobiologiia; 1981; 50(5):891-7. PubMed ID: 7033736.
    Abstract:
    The release of proteins was studied during rehydration of Saccharomyces cerevisiae cells dehydrated using two techniques which were characterized by different survival rate. A correlation was established between the loss of proteins and the survival rate of cells after dehydration and rehydration. Proteins with a molecular mass from 6000 to 70,000 daltons (low molecular weight proteins predominating) were found in the rehydration medium by the method of disc-electrophoresis in polyacrylamide gel in the presence of sodium dodecylsulfate. Moreover, the rehydration medium exhibited the activity of certain phosphohydrolases and dehydrogenases; this finding suggests that proteins with a higher molecular weight are present in the medium.
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