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  • Title: Tritium labeling of gonadotropin releasing hormone in its proline and histidine residues.
    Author: Klauschenz E, Bienert M, Egler H, Pleiss U, Niedrich H, Nikolics K.
    Journal: Peptides; 1981; 2(4):445-52. PubMed ID: 7036106.
    Abstract:
    3,4-dehydroproline9-GnRH prepared by solid phase peptide synthesis was tritiated catalytically under various conditions yielding 3H-GnRH with specific radioactivities in the range from 35-60 Ci/mmol and full LH releasing activity in vitro. Using palladium/alumina catalyst, the tritiation of the double bond occurs within ten minutes. Investigation of the tritium distribution between the amino acid residues showed a remarkably high incorporation of tritium into the histidine residue (11 to 37%). On the basis of this observation, the tritium labeling of GnRH and angiotensin I by direct catalytic hydrogen-tritium exchange was found to be useful for the labeling of these peptides at remarkably high specific radioactivity.
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