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  • Title: [Growth of Micrococcus lysodeikticus bacteria on a deuterated medium].
    Author: Eremin VA, Chekulaeva LN, Kharat'ian EF, Ostrovskiĭ DN.
    Journal: Mikrobiologiia; 1978; 47(4):629-36. PubMed ID: 703645.
    Abstract:
    The object of this work was to prepare deuterated growth media and to adapt Micrococcus lysodeikticus to a medium containing deuterated-substituted organic substances and deuterium oxide instead of water. M. lysodeikticus was grown on a medium prepared from the "deuterated-cells" of Chlorella, and was capable of absorbing selectively protons from such a medium containing high concentrations of deuterium. Its deuterated cells ("monsters") produced structures consisting of several (up to 8) smaller cells, angular in shape and having a thicker (2--3 times) cell wall. Apparently, adaptation to a deuterated medium is accompanied with changes in the cell wall biosynthesis as a result of which the separation of daughter cells is interfered with in the course of cell division, and the cells are more resistant to the action of lysozyme.
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