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  • Title: Some characteristics of tetraphenylphosphonium uptake into Saccharomyces cerevisiae.
    Author: Boxman AW, Barts PW, Borst-Pauwels GW.
    Journal: Biochim Biophys Acta; 1982 Mar 23; 686(1):13-8. PubMed ID: 7039677.
    Abstract:
    The characteristics of the uptake of the lipophilic cation tetraphenylphosphonium (TPP+) into Saccharomyces cerevisiae have been investigated in order to establish whether this compound can be used to monitor the membrane potential of his organism. Unlike dibenzyldimethylammonium, TPP+ is not translocated via the thiamine transport system, nor via another inducible translocation mechanism. On changing the experimental conditions the equilibrium potential of TPP+ varies according to expected changes of the membrane potential. TPP+ accumulation is higher in metabolizing cells than in non-metabolizing cells. In addition, decreasing the medium pH, addition of the proton conductor 2,4-dinitrophenol and addition of K+ all cause an apparent depolarization, whereas Ca2+ apparently hyperpolarizes the cell membrane. It is concluded that TPP+, if applied at low concentrations, can be used to measure the membrane potential of S. cerevisiae.
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