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  • Title: Differentiation of Haemophilus spp. in Respiratory isolate cultures by an indole spot test.
    Author: Welch DF, Ahlin PA, Matsen JM.
    Journal: J Clin Microbiol; 1982 Feb; 15(2):216-9. PubMed ID: 7040445.
    Abstract:
    Indole spot tests using isolated, nonhemolytic colonies of Haemophilus species were positive for 90 of 151 (60%) respiratory isolates of Haemophilus influenzae, whereas 67 to 72 (93%) isolates of H. influenzae from cerebrospinal fluid and blood specimens were indole positive. Only 4 of 117 (3%) Haemophilus parainfluenzae isolates were positive for indole spot tests. Thus, indole-positive, nonhemolytic Haemophilus isolates in respiratory cultures can be presumptively identified as H. influenzae.
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