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  • Title: The use of paraffin-processed tissue and the immunoperoxidase technique in the diagnosis of bullous diseases, lupus erythematosus and vasculitis.
    Author: Turbitt ML, Mackie RM, Young H, Campbell I.
    Journal: Br J Dermatol; 1982 Apr; 106(4):411-7. PubMed ID: 7041947.
    Abstract:
    Twenty-two skin biopsies have been split and each half examined either by the immunoperoxidase technique on paraffin-fixed tissue or by both immunoperoxidase (IP) and immunofluorescence (IF) technique on snap-frozen tissue. All twenty-two specimens gave positive results by frozen IF techniques, nineteen out of twenty-two (86%) were positive by frozen IP techniques and sixteen out of twenty-two (72%) were positive by IP on paraffin-fixed material. If retrospective immunopathology is required on a specimen and only paraffin-fixed material is available a positive result may be expected in the majority of cases.
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