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  • Title: [Horseradish peroxidase: kinetic studies and optimization of the activity determination with the substrates H2O2 and o-phenylenediamine (author's transl)].
    Author: Gallati H, Brodbeck H.
    Journal: J Clin Chem Clin Biochem; 1982 Apr; 20(4):221-5. PubMed ID: 7042898.
    Abstract:
    To optimize the determination of the activity of peroxidase, an enzyme often used as a marker and indicator system in enzyme immunoassays, the kinetics of the catalytic reaction and its dependence on the H2O2 and o-phenylenediamine concentration at various pH values were investigated. In addition, the influence of buffer systems, the reaction temperature, and the reaction time were determined. Based on the results, a test procedure is proposed for the measurement of peroxidase down to concentrations of 2 ng/l.
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