These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A simple, rapid and reliable enzyme-linked immunosorbent assay (ELISA) for measuring estrone-3-glucuronide in urine.
    Author: Shah HP, Joshi UM.
    Journal: J Steroid Biochem; 1982 Feb; 16(2):283-6. PubMed ID: 7043092.
    Abstract:
    A rapid, simple and sensitive enzyme-linked immunosorbent assay for the estimation of estrone-3-glucuronide (E1G) in diluted unprocessed urine has been developed using E1G-penicillinase (E.C.3.5.2.6.) prepared via carbodiimide linkage. Polyvinyl chloride ELISA plate (Dynatech) coated with antibody was used for separation of the antibody bound ligand. A complete assay could be performed within 4 hours with good precision (C.V. less than 17%). Minimum detectable amount of E1G was 25 pg/well. Parallelism was observed between sample dilution curve and standard curve. Recovery of E1G from urine ranged between 85-105%. Results obtained on 29 urine samples by the ELISA procedure were comparable to those obtained with an established RIA procedure (r = 0.96). The present method may thus provide a useful tool for the assessment of ovarian function.
    [Abstract] [Full Text] [Related] [New Search]