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  • Title: [Optimization of the composition of the medium for thrombolytic enzyme biosynthesis in pure and mixed cultures].
    Author: Gesheva VI, Maksimov VN, Landau NS, Egorov NS.
    Journal: Mikrobiologiia; 1982; 51(2):206-11. PubMed ID: 7045597.
    Abstract:
    The medium for the biosynthesis of exoproteases with the thrombolytic action in monocultures and mixed cultures of actinomycetes was optimized in several steps using the method of complete and fractional factor experiments. When Actinomyces rimosus was cultivated on the selected medium, the productivity of the mycelium rose, the fibrinolytic activity increased three-fold, and the caseinolytic activity became 2.2 times greater. During mixed cultivation of A. rimosus and A. violaceus on this medium, fibrinolysis increased six times comparing with the A. rimosus monoculture growth on the original medium while caseinolysis became only 3.7 times greater, which increased the specificity for fibrin twice.
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