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  • Title: Isolation of genome-enzyme complex from cytoplasmic polyhedrosis virus of silkworm Bombyx mori.
    Author: Dai R, Wu A, Shen X, Qian L, Sun Y.
    Journal: Sci Sin B; 1982 Jan; 25(1):29-35. PubMed ID: 7048527.
    Abstract:
    The genome-enzyme complex is isolated from the silkworm cytoplasmic polyhedrosis virus with DEAE-Sephadex column chromatography after ultraviolet irradiation. The genome-enzyme complex shows both RNA-polymerase and methyltransferase activities while using 3H-UTP and [3H-methyl]-S-adenosyle-L-methionine as substrates. Like the double-stranded RNA genome of CPV, the genome-enzyme complex could be separated into nine segments on polyacrylamide gel electrophoresis. It is demonstrated that the RNA polymerase and methyl-transferase are tightly bound to the double-stranded RNA genome and that the individual segments of the genome-enzyme complex all possess RNA-polymerase and methyltransferase activities. It appears that each segment of the double-stranded RNA genome is transcribed independently.
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